hcs77

Figure 9 HCS77‐deficient cells are defective in heat induction of Mpk1 kinase activity. ragam4d a/α HCS77. flas4d HCS77 (JO227) and a/α hcs77Δ hcs77Δ (JO347) strains were transformed with pDL922 expressing Mpk1HA Transformants were grown at 25°C in Ura drop‐out medium and shifted to 39°C for 30 min Extracts were prepared from each sample and